Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

• DOI: 10.1021/acssensors.8b00048
• Publication Date: 2018-05-31T11:14:06Z
• Authors: Bo Tian; Xiaoqi Liao; Peter Svedlindh; Mattias Strömberg; Erik Wetterskog
• Keywords: Superparamagnetism

The ability to detect and analyze the state of magnetic labels with high sensitivity is crucial importance for developing biosensors. In this work, we demonstrate, first time, a ferromagnetic resonance (FMR) based homogeneous volumetric biosensor label detection. Two different isothermal amplification methods, i.e., rolling circle (RCA) loop-mediated (LAMP), are adopted combined standard electron paramagnetic (EPR) spectrometer FMR biosensing. For RCA-based biosensor, binding RCA products synthetic Vibrio cholerae target DNA sequence gives rise formation aggregates nanoparticles. Immobilization nanoparticles within leads decrease net anisotropy system concomitant increase field. A limit detection 1 pM obtained linear range between 7.8 250 pM. LAMP-based sensing, Zika virus oligonucleotide amplified detected in 20% serum samples. induced by their coprecipitation Mg2P2O7 (a byproduct LAMP) provides 100 aM. fast measurement, sensitivity, miniaturization potential proposed biosensing technology makes it promising candidate designing future point-of-care devices.