Plasmonic Nanosensor Array for Multiplexed DNA-based Pathogen Detection

• DOI: 10.1021/acssensors.8b01073
• Publication Date: 2019-01-18T15:51:32Z
• Authors: David Zopf; Angelina Pittner; André Dathe; Norman Grosse; Andrea Csáki; Kai Arstila; J. Jussi Toppari; Walter Schott; Denis Dontsov; Günter Uhlrich; Wolfgang Fritzsche; Ondrej Stranik
• Keywords: ta221; Metal Nanoparticles; 02 engineering and technology; biosensorit; Accelerator Laboratory; Limit of Detection; plasmonic array; Nanotechnology; LSPR sensing; DNA, Fungal; Candida; DNA detection; ta114; Base Sequence; Fourier-transform-imaging spectroscopy; Kiihdytinlaboratorio; ta1182; nanobiotekniikka; DNA; Surface Plasmon Resonance; plasmonic nanoparticles; Aspergillus; Nanoscience Center; nanohiukkaset; Gold; DNA Probes; 0210 nano-technology

In this research we introduce a plasmonic nanoparticle based optical biosensor for monitoring of molecular binding events. The sensor utilizes spotted gold nanoparticle arrays as sensing platform. The nanoparticle spots are functionalized with capture DNA sequences complementary to the analyte (target) DNA. Upon incubation with the target sequence, it will bind on the respectively complementary functionalized particle spot. This binding changes the local refractive index, which is detected spectroscopically as the resulting changes of the localized surface plasmon resonance (LSPR) peak wavelength. In order to increase the signal, a small gold nanoparticle label is introduced. The binding can be reversed using chemical means (10 mM HCl). It is demonstrated that multiplexed detection and identification of several fungal pathogen DNA sequences subsequently on one sensor array are possible by this approach.