Rapidly Visualizing the Membrane Affinity of Gene Vectors Using Polydiacetylene-Based Allochroic Vesicles

• DOI: 10.1021/acssensors.9b00102
• Publication Date: 2019-03-14T13:25:42Z
• Authors: Jing-Wen Wang; Feng Zheng; Huan Chen; Ya Ding; Xing-Hua Xia
• Keywords: 0301 basic medicine; Cell Membrane; Genetic Vectors; Green Fluorescent Proteins; Static Electricity; Gene Transfer Techniques; Phosphatidylglycerols; 01 natural sciences; Polyacetylene Polymer; 0104 chemical sciences; 03 medical and health sciences; Cell Line, Tumor; Liposomes; Humans; Colorimetry; Dimyristoylphosphatidylcholine; Stearic Acids

The high-throughput screening of chemically active substances has aroused widespread interest in recent years. drug carriers is usually ignored, although they interact directly with physiological barriers and target cells, determine the fate efficacy drugs vivo. In this work, a series polydiacetylene (PDA) vesicles (ca. 550 nm) that simulate cell membrane are constructed to detect affinity gene vectors. surface potentials adjusted by changing phospholipid composition using different charged compounds. All show rapid color changes upon addition vectors naked eye within <5 min. optimized 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC)-PDA display most sensitive discoloration response commercially available vectors, including Lipofectamine 2000, Entranster-H4000, polyethylenimine. logarithm transfection efficiency for green fluorescent protein plasmid (pGFP) mediated these three L02 HepG2 cells demonstrate an excellent linear correlation (log Kb) detected DMPC-PDA vesicles. This visualization method not only allows vitro prediction greatly contributes efficiency, but also offers universal strategy potential various carrier materials featuring high affinity.