Nanopore-Based Strategy for Sensing of Copper(II) Ion and Real-Time Monitoring of a Click Reaction

• DOI: 10.1021/acssensors.9b00236
• Publication Date: 2019-05-03T12:00:28Z
• Authors: Liping Liu; Zhen Fang; Xiangjiang Zheng; Dongmei Xi
• Keywords: Nanopore; SIGNAL (programming language)

A straightforward yet efficient, nanopore-based strategy that enables the sensitive detection of copper(II) ion (Cu2+) and real-time monitoring a click reaction is provided. Two single-stranded DNAs (ssDNAs) are designed to act as preprobes, one being modified with an azide other alkyne. The presence Cu2+ induces ligation two ssDNAs via reaction, leading formation forked DNA which can quantitatively generate characteristic current signatures when interacts α-hemolysin (α-HL) nanopore. assay facilitates highly selective measurement without need for labels or signal amplification. More importantly, this nanopore platform exhibits excellent performance in copper(I) (Cu+)-catalyzed at single-molecule level, by recording signals generated chemistry. proposed believed play important role both sensing characterization chemistry reactions, especially coupling reactions.