Rapid Detection of Circulating Breast Cancer Cells Using a Multiresonant Optical Fiber Aptasensor with Plasmonic Amplification

• DOI: 10.1021/acssensors.9b02155
• Publication Date: 2020-01-22T14:09:45Z
• Authors: Médéric Loyez; Eman M. Hassan; Maxime Lobry; Fu Liu; Christophe Cauche...; Ruddy Wattiez; Maria C. DeRosa; William G. Willmore; Jacques Albert
• Keywords: Circulating tumor cell; Aptamer; Mammaglobin; Cancer Detection

The detection of circulating tumor cells (CTCs), which are responsible for metastasis in several forms cancer, represents an important goal oncological diagnosis and treatment. These remain extremely challenging to detect, despite numerous previous studies, due their low concentration (1–10 cells/mL blood). In this work, all-fiber plasmonic aptasensor featuring multiple narrowband resonances the near-infrared wavelength range was developed detect metastatic breast cancer cells. To aim, specific aptamers against mammaglobin-A were selected immobilized as receptors on sensor surface. vitro assays confirm that label-free real-time [limit (LOD) 49 cells/mL] occurs within 5 min, while additional use functionalized gold nanoparticles allows a 2-fold amplification biosensor response. Differential measurements optical used process response, results confirmed by microscopy. only 10 achieved with relevant specificity control quick response time.