Engineering a Central Carbon Metabolism Pathway to Increase the Intracellular Acetyl-CoA Pool in Synechocystis sp. PCC 6803 Grown under Photomixotrophic Conditions

• DOI: 10.1021/acssynbio.0c00629
• Publication Date: 2021-03-29T15:46:40Z
• Authors: Xinyu Song; Jinjin Diao; Jiaqi Yao; Jinyu Cui; Tao Sun; Lei Chen; Weiwen Zhang
• Keywords: Synechocystis; Metabolic Engineering; Metabolic pathway

In cyanobacteria, photomixotrophic growth is considered as a promising strategy to achieve both high cell density and product accumulation. However, the conversion of glucose acetyl coenzyme A (acetyl-CoA) in native glycolytic pathway insufficient, which decreases carbon utilization productivity engineered cyanobacteria under conditions. To increase flux from key intracellular precursor acetyl-CoA Synechocystis sp. PCC 6803 (hereafter, 6803) conditions, synthetic nonoxidative cyclic glycolysis (NOG) was introduced into wild type strain, successfully increased pool by approximately 1-fold. minimize competition for glucose, Embden-Meyerhof-Parnas (EMP) Entner-Doudoroff (ED) pathways were knocked out, respectively. Notably, eliminating ED strain carrying NOG further up 2.8-fold. Another consuming 6803, glycogen biosynthesis pathway, additionally out above-mentioned enabled an 3.5-fold when compared with strain. Finally, content lipids analyzed index productive capacity factory The results showed total yield about 26% (from 15.71% 34.12%, g/g glucose), demonstrating that this integrated approach could represent general not only improvement concentration acetyl-CoA, but also production value-added chemicals require cyanobacteria.