Metabolic Engineering of E. coli for Xylose Production from Glucose as the Sole Carbon Source

• DOI: 10.1021/acssynbio.1c00184
• Publication Date: 2021-08-20T03:29:54Z
• Authors: Wencheng Yin; Yujin Cao; Miaomiao Jin; Mo Xian; Wei Liu
• Keywords: Xylose isomerase; Metabolic Engineering; Xylose metabolism; Downstream processing; Metabolic pathway

Xylose is the raw material for synthesis of many important platform compounds. At present, xylose commercially produced by chemical extraction. However, there are still some bottlenecks in extraction xylose, including complicated operation processes and substances introduced, leading to high cost synthesizing downstream compounds xylose. The current market price 8× that glucose, so using low-cost glucose as substrate produce can theoretically reduce 70%. Here, we designed a pathway biosynthesis from Escherichia coli. This biosynthetic was achieved overexpressing five genes, namely, zwf, pgl, gnd, rpe, xylA, while replacing native xylulose kinase gene xylB with araL B. subtilis, which displays phosphatase activity toward d-xylulose 5-phosphate. yield increased 3.3 g/L optimizing metabolic pathway. Furthermore, xylitol successfully synthesized introducing xyl1 gene, suggested universally applicable first study synthesize its substrate, not only reduces materials, but also alleviates carbon catabolite repression (CCR), providing new idea