Engineering an Efficient Expression Using Heterologous GAL Promoters and Transcriptional Activators in Saccharomyces cerevisiae

• DOI: 10.1021/acssynbio.3c00243
• Publication Date: 2023-05-24T18:08:50Z
• Authors: Yanling Wu; Jiliang Deng; Zhaohui Zheng; Nanzhu Chen; Xiaozhou Luo; Hongting Tang
• Keywords: Heterologous; Kluyveromyces lactis; Heterologous expression; Transcription

Galactose-inducible (GAL) promoters have been widely used in metabolic engineering Saccharomyces cerevisiae for production of valuable products. Endogenous GAL and transcription factors often engineered to improve promoter activities. Heterologous activator (Gal4p-like transcriptional activators), although existing other yeasts or fungi, not well explored. In this study, we comprehensively characterized the activation effects Gal4p activators from different fungi on a variant promoters. Overexpressing endogenous driven by PHHF1 increased activities native PGAL1 heterologous PSkGAL2 131.20% 72.45%, respectively. Furthermore, eight organisms were most them exhibited functions that consistent with ScGal4p. Expression KlLac9p Kluyveromyces lactis further activity PScGAL1 41.56% 100.63%, respectively, compared ScGal4p expression, was able evade Gal80p inhibition. This optimized expression system can be increase β-carotene 9.02-fold S. cerevisiae. Our study demonstrated combination provided novel insights into optimization system.