AutoBioTech─A Versatile Biofoundry for Automated Strain Engineering

• DOI: 10.1021/acssynbio.4c00298
• Publication Date: 2024-07-08T12:23:47Z
• Authors: Tobias Michael Rosch; Julia Tenhaef; Tim Stoltmann; Till Redeker; Dominic Kösters; Niels Hollmann; Karin Krumbach; Wolfgang Wiechert; Michael Bott; Susana Matamouros; Jan Marienhagen; Stephan Noack
• Keywords: Corynebacterium glutamicum; Genome Engineering; Toolbox; Cloning (programming); Modularity; Synthetic Biology

The inevitable transition from petrochemical production processes to renewable alternatives has sparked the emergence of biofoundries in recent years. Manual engineering microbes will not be sufficient meet ever-increasing demand for novel producer strains. Here we describe AutoBioTech platform, a fully automated laboratory system with 14 devices perform operations strain construction without human interaction. Using modular workflows, this platform enables transformations Escherichia coli plasmids assembled via cloning. A CRISPR/Cas9 toolbox compatible existing cloning frameworks allows and flexible genome editing E. coli. In addition, workflows have been established transformation Gram-positive model organism Corynebacterium glutamicum by conjugation electroporation, latter proving more robust technique. Overall, excels at versatility due modularity seamless transitions between modules. This accelerate Gram-negative bacteria.