Synthetic Toolkit for Complex Genetic Circuit Engineering in Saccharomyces cerevisiae

• DOI: 10.1021/acssynbio.8b00076
• Publication Date: 2018-05-11T16:46:44Z
• Authors: Anssi Rantasalo; Joosu Kuivanen; Merja Penttilä; Jussi Jäntti; Dominik Mojzita
• Keywords: Synthetic Biology; Metabolic Engineering; Gene regulatory network; Modularity; Heterologous

Sustainable production of chemicals, materials, and pharmaceuticals is increasingly performed by genetically engineered cell factories. Engineering complex metabolic routes or behavior control systems requires robust predictable gene expression tools. In this challenging task, orthogonality a fundamental prerequisite for such study, we developed characterized in depth comprehensive toolkit that allows accurate Saccharomyces cerevisiae without marked interference with native cellular regulation. The comprises set transcription factors, designed to function as synthetic activators repressors, transcription-factor-dependent promoters, which together provide broad range surpassing, at high end, the strongest promoters. Modularity tools demonstrated establishing novel bistable genetic circuit performance heterologous pathway enabling on-demand switching between two alternative branches.