Ribulose Monophosphate Shunt Provides Nearly All Biomass and Energy Required for Growth of E. coli

• DOI: 10.1021/acssynbio.8b00093
• Publication Date: 2018-05-14T13:42:00Z
• Authors: Hai He; Christian Edlich-...; Steffen N. Lindner; Arren Bar-Even
• Keywords: Rump; Ribulose

The ribulose monophosphate (RuMP) cycle is a highly efficient route for the assimilation of reduced one-carbon compounds. Despite considerable research, RuMP has not been fully implemented in model biotechnological organisms such as Escherichia coli, mainly since heterologous establishment pathway requires addressing multiple challenges: sufficient formaldehyde production, assimilation, and regeneration acceptor, 5-phosphate. Here, by efficiently producing from sarcosine oxidation 5-phosphate exogenous xylose, we set aside two these concerns, allowing us to focus on particular challenge establishing via shunt, linear variant cycle. We have generated deletion strains whose growth depends, different extents, activity thus incrementally increasing selection pressure synthetic pathway. Our final strain depends shunt providing cell with almost all biomass energy needs, presenting an absolute coupling between key components. This study shows value stepwise problem solving approach when difficult but promising pathway, strong basis future engineering, selection, evolution