An Expanded Synthetic Biology Toolkit for Gene Expression Control in Acetobacteraceae

• DOI: 10.1021/acssynbio.8b00168
• Publication Date: 2019-03-13T22:18:06Z
• Authors: Min Yan Teh; Kean Hean Ooi; Shun Xiang Danny Teo; Mohammad Ehsan Bi...; Wen Zheng Shaun Lim; Meng How Tan
• Keywords: Synthetic Biology; Bacterial Cellulose; Polyhydroxybutyrate

The availability of different host chassis will greatly expand the range applications in synthetic biology. Members Acetobacteraceae family Gram-negative bacteria form an attractive class nonmodel microorganisms that can be exploited to produce industrial chemicals, food and beverage, biomaterials. One such biomaterial is bacterial cellulose, which a strong ultrapure natural polymer used tissue engineering scaffolds, wound dressings, electronics, additives, other products. However, despite potential biotechnology, there has been considerably little effort fundamentally reprogram for enhanced performance. limiting factor lack well-characterized, comprehensive toolkit control expression genes biosynthetic pathways regulatory networks optimize production cell viability. Here, we address this shortcoming by building expanded genetic biology Acetobacteraceae. We characterized performance multiple promoters, ribosome binding sites, terminators, degradation tags three strains, namely, Gluconacetobacter xylinus ATCC 700178, hansenii 53582, Komagataeibacter rhaeticus iGEM. Our quantitative data revealed strain-specific common design rules precise gene these industrially relevant species. further applied our tools synthesize biodegradable cellulose-chitin copolymer, adjust structure cellulose film produced, implement CRISPR interference ready down-regulation expression. Collectively, parts enable efficient biomanufacturing cellulose-based materials commercially valuable