Genome-Wide CRISPRi-Based Identification of Targets for Decoupling Growth from Production

• DOI: 10.1021/acssynbio.9b00143
• Publication Date: 2020-04-08T22:56:11Z
• Authors: Songyuan Li; Christian Bille J...; Jenny Landberg; Lasse Ebdrup Pede...; Nikolaus Sonnensc...; Sheila Ingemann J...; Alex Toftgaard Ni...
• Keywords: Decoupling (probability); Synthetic Biology

Growth decoupling can be used to optimize microbial production of biobased compounds by inhibiting excess biomass formation and redirect carbon flux from growth product formation. However, identifying suitable genetic targets through rational design is challenging. Here, we conduct a genome-wide CRISPRi screen discover switches for production. Using an sgRNA library covering 12 238 loci in the Escherichia coli genome, that inhibit while allowing continued protein In total, identify 1332 sgRNAs simultaneously decrease maintain or increase accumulation GFP. The top target sibB/ibsB shows more than 5-fold GFP 45% Overall, our provides key decoupling, approach applied improve other microorganisms.