Design and Characterization of Biosensors for the Screening of Modular Assembled Naringenin Biosynthetic Library in Saccharomyces cerevisiae
Metabolic Engineering
Synthetic Biology
DOI:
10.1021/acssynbio.9b00212
Publication Date:
2019-08-21T19:57:06Z
AUTHORS (8)
ABSTRACT
A common challenge in the assembly and optimization of plant natural product biosynthetic pathways recombinant hosts is identification gene orthologues that will result best production titers. Here, we describe modular a naringenin pathway Saccharomyces cerevisiae was facilitated by optimized naringenin-inducible prokaryotic transcription activators used as biosensors. The biosensors were designed developed S. multiparametric engineering strategy, which further applied for vivo, high-throughput screening established yeast library. workflow assembling involved Golden gate-directed combinatorial genes promoters, resulting strain library ideally covering 972 combinations cerevisiae. For improving performance our biosensor, series fundamental components optimized, affecting efficiency biosensor such nuclear localization signal (NLS), detector module effector module. One (pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1-MV2) showed better performance, defined dynamic range sensitivity than others this study well other previously reported Using able to identify most efficient candidate from This approach can be exploited metabolites derived flavonoid more importantly employed characterization putative genes.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (41)
CITATIONS (56)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....