Production of Biocompatible and Antimicrobial Bacterial Cellulose Polymers Functionalized by RGDC Grafting Groups and Gentamicin

• DOI: 10.1021/am4027983
• Publication Date: 2014-01-08T01:44:45Z
• Authors: Mahmoud Rouabhia; Jérémie Asselin; Neftaha Tazi; Younès Messaddeq; Dennis Levinson; Ze Zhang
• Keywords: Surface Modification; Bacterial Cellulose; Biocompatibility

Bacterial cellulose (BC), a three-dimensional fibril, is natural polymer that can be used for many applications. BC effectiveness may improved by enhancing surface characteristics contributing to better physiologic interaction with human and animal cells intrinsically present antimicrobial agents. In the study, gentamicin-activated membranes were obtained chemically grafting RGDC peptides (R: arginine; G: glycine; D: aspartic acid; C: cysteine) using coupling agent 3-aminopropyltriethoxysilane (APTES) followed covalent attachment of gentamicin onto membrane network. X-ray photoelectron spectroscopy (XPS) analyses showed BC–APTES contained 0.7% silicon in terms elemental composition, corresponding ratio 1:12. The presence nitrogen confirmed functionalization membrane. Fourier-transform infrared (FTIR) show formation secondary amide as supported valence bond C═O (νC═O), characteristic vibrational transition at 1650 cm–1 which particularly intense BC–RGDC–gentamicin Energy-dispersive (EDX) low level carbon (C + N) pure but high confirming modification enrichment. Of great interest, gentamicin–RGDC-grafted are bactericidal against Streptococcus mutans nontoxic dermal fibroblasts thus useful multiple applications such wound healing drug delivery systems.