Facile Synthesis of Guanidyl-Functionalized Magnetic Polymer Microspheres for Tunable and Specific Capture of Global Phosphopeptides or Only Multiphosphopeptides

• DOI: 10.1021/am506882b
• Publication Date: 2014-12-03T11:37:52Z
• Authors: Zhichao Xiong; Yajing Chen; Lingyi Zhang; Jun Ren; Quanqing Zhang; Mingliang Ye; Weibing Zhang; Hanfa Zou
• Keywords: Phosphopeptides; Polymers; 01 natural sciences; Microspheres; 0104 chemical sciences; Magnetic Fields; Polymethacrylic Acids; Materials Testing; Particle Size; Crystallization; Magnetite Nanoparticles; Guanidine

The highly selective and efficient capture of heterogeneous types phosphopeptides is critical for comprehensive in-depth phosphoproteome analysis, but it still remains a challenge since the lack affinity material with large binding capacity controllable specificity. Here, new was prepared to improve enrichment endue tunable specificity by introducing guanidyl onto poly(glycidyl methacrylate) (PGMA) modified Fe3O4 microsphere (denoted as Fe3O4@PGMA-Guanidyl). thick polymer shell endows composite amount beneficial enhancing interaction between material. Interestingly, Fe3O4@PGMA-Guanidyl possesses enriching ability global or only multiphosphopeptides through simple regulation buffer composition. has (200 mg g–1), extremely high detection sensitivity (0.5 fmol), recovery (91.30%), great specificity, rapid magnetic separation. Moreover, result application from tryptic digest nonfat milk demonstrated potential in identification low-abundance interest biological sample.