Supramolecular Assemblies of Histidinylated α-Cyclodextrin in the Presence of DNA Scaffold during CDplexes Formation

• DOI: 10.1021/bc200167p
• Publication Date: 2011-10-21T06:14:42Z
• Authors: Véronique Benneva...; Allan Urbach; Olivier Martin; Chantal Pichon; Philippe Guégan; Patrick Midoux
• Keywords: DNA condensation; Polylysine; Imidazole; Cationic polymerization

α-Cyclodextrin was transformed in a cationic unit after per substitution with histidine (His-α-CD) and lysine (Lys-α-CD) molecules on the primary face. His-α-CD Lys-α-CD were used to form electrostatic complexes (CDplexes) plasmid DNA encoding luciferase gene, ability of CDplexes transfect mammalian cells examined using HEK293-T7 cells. The activity transfected His-α-CDplexes 8-fold higher than that obtained Lys-α-CDplexes. When transfection carried out presence chloroquine, Lys-α-CDplexes increased 6 25 times, respectively. lower enhancement confirmed induced proton sponge effect inside endosomes upon imidazole protonation, favoring delivery cytosol. At same time, we found condensation unexpectedly stronger lysyl-α-CD counterpart. Moreover, it as strong observed high molecular weight polylysine. NMR (ROESY DOSY) investigations absence showed an inclusion complex is formed between ring hydrophobic cavity CD but no polymers can be by intermolecular interactions. These results suggest interactions could involved supramolecular assemblies scaffold leading into low diameter particles.