A Bioluminescent Probe for Salivary Cortisol
0303 health sciences
Binding Sites
Hydrocortisone
Enzyme-Linked Immunosorbent Assay
Sensitivity and Specificity
3. Good health
03 medical and health sciences
Receptors, Glucocorticoid
Genes, Reporter
COS Cells
Chlorocebus aethiops
Luminescent Measurements
Animals
Humans
Luciferases
Promoter Regions, Genetic
Saliva
Glucocorticoids
DOI:
10.1021/bc200220k
Publication Date:
2011-08-22T12:09:34Z
AUTHORS (3)
ABSTRACT
Cortisol is a classical biomarker for the stress levels of human beings. We fabricated highly sensitive bioluminescent probes salivary cortisol. The following strategies were contrived in molecular design. Gaussia princeps luciferase (GLuc) was dissected into two fragments, between which an N-terminal-extended ligand binding domain glucocorticoid receptor (GR HLBD), named Simgr4, inserted. First, this unique single-chain probe then situated downstream response element (GRE) promoter reporter-gene system constructing ON–OFF switches Second, circularly permutated (CP) variant Simgr4 formulated. exerted improved signal-to-background (S/B) ratio 8.5 to Furthermore, 10× enhanced detection limit cortisol and long dynamic range from 10–9 10–6 M cortisol, covering all normal clinical ranges serum, urine, saliva. This optimized successfully determined daily fluctuations correlations with those by ELISA. study first investigate contribution HLBD nuclear multiple cortisols.
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CITATIONS (27)
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