In Vitro Analysis of Carboxyacyl Substrate Tolerance in the Loading and First Extension Modules of Borrelidin Polyketide Synthase

• DOI: 10.1021/bi500951c
• Publication Date: 2014-09-04T20:06:18Z
• Authors: Andrew Hagen; Sean Poust; Tristan de Rond; Satoshi Yuzawa; Leonard Katz; Paul D. Adams; Christopher J. Pe...; Jay D. Keasling
• Keywords: Polyketide synthase; Moiety

The borrelidin polyketide synthase (PKS) begins with a carboxylated substrate and, unlike typical decarboxylative loading PKSs, retains the carboxy group in final product. specificity and tolerance of incorporation carboxyacyl into type I PKSs have not been explored. Here, we show that first extension module is promiscuous its ability to extend both non-carboxyacyl substrates. However, has requirement for substrates containing moiety, which are decarboxylated situ. Thus, basis observed specific starter units by borelidin PKS.