Preparation of Thermoresponsive Cationic Copolymer Brush Surfaces and Application of the Surface to Separation of Biomolecules

• DOI: 10.1021/bm701427m
• Publication Date: 2008-03-22T05:01:10Z
• Authors: Kenichi Nagase; Jun Kobayashi; Akihiko Kikuchi; Yoshikatsu Akiyama; Hideko Kanazawa; Teruo Okano
• Keywords: Cationic polymerization; Atom-transfer radical-polymerization

We have prepared poly(N-isopropylacrylamide (IPAAm)-co-2-(dimethylamino)ethylmethacrylate (DMAEMA)) brush-grafted silica bead surfaces through surface-initiated atom transfer radical polymerization (ATRP) using the CuCl/CuCl2/Me6TREN catalytic system in 2-propanol at 25 °C for 16 h. The temperature-responsive were characterized by chromatographic analysis modified beads as stationary phases. Chromatographic retention times adenosine nucleotides aqueous mobile phases significantly increased compared to that previously reported other cationic hydrogel surfaces, indicating strong electrostatic copolymer brush interactions occur between and nucleotide analytes. Retention decreased with increasing column temperature, explained decreasing basicity temperature. Step-temperature gradients from 10 50 shorten ATP times. These results indicate ATRP can rapidly alter their properties changing