Polycyclic Aromatic Hydrocarbons-Associated MicroRNAs and Their Interactions with the Environment: Influences on Oxidative DNA Damage and Lipid Peroxidation in Coke Oven Workers

• DOI: 10.1021/es4055516
• Publication Date: 2014-02-20T18:29:34Z
• Authors: Qifei Deng; Xiayun Dai; Huan Guo; Suli Huang; Dan Kuang; Jing Feng; Tian Wang; Wangzhen Zhang; Kun Huang; Die Hu; Huaxin Deng; Xiaomin Zhang; Tangchun Wu
• Keywords: DNA adduct

We previously identified five polycyclic aromatic hydrocarbons (PAHs)-associated microRNAs (miRNAs) and found they were associated with chromosome damage. As oxidative damage is the common contributory cause of various PAHs-related diseases, we further investigated influences these miRNAs their interactions environmental factors on DNA lipid peroxidation. measured PAHs internal exposure biomarkers [urinary monohydroxy-PAHs (OH-PAHs) plasma benzo[a]pyrene-r-7,t-8,t-9,c-10-tetrahydotetrol-albumin (BPDE-Alb) adducts], expression levels PAHs-associated (miR-24-3p, miR-27a-3p, miR-142-5p, miR-28-5p, miR-150-5p), urinary [8-hydroxydeoxyguanosine (8-OH-dG)] peroxidation [8-iso-prostaglandin-F2α (8-iso-PGF2α)] in 365 healthy male coke oven workers. These a dose–response increase 8-OH-dG (β > 0), decrease 8-iso-PGF2α < especially workers lower levels, nonsmokers, nondrinkers. interacted antagonistically ΣOH-PAHs BPDE-Alb adducts (βinteraction 0) synergistically drinking status to influence 8-OH-dG, while smoking 8-iso-PGF2α. Our results suggested that might be novel mechanisms mediating effects