Occurrence of Two Genotypes of Tetracycline (TC) Resistance Gene tet(M) in the TC-Resistant Bacteria in Marine Sediments of Japan

• DOI: 10.1021/es702986y
• Publication Date: 2008-06-17T07:01:41Z
• Authors: M. Habibur Rahman; Lisa Nonaka; Ryosuke Tago; Satoru Suzuki
• Keywords: Marine bacteriophage; Strain (injury)

The tetracycline (TC) resistance gene tet(M) was monitored in bacteria isolated from Japanese coastal and off-shore marine sediments. high rate of occurrence TC resistant (TCr) (120 µg mL−1 TC) observed at frequency ranges between 0.0−0.08% Tokyo Bay, 1.67−1.82% Sagami Bay 0.0−4.35% the open Pacific Ocean. PCR amplified TCr isolates, showing 127 209 isolates (60.8%) as positive. 32.0−96.0%, 21.1 −28.0% 0.0−83.3% Ocean, respectively. positive belonged to 4 orders bacteria. Bacillales most dominant order (121 strains) among possessing bacteria, followed by Actinomycetales (three strains), Flavobacteriales (one strain) Pseudomonadales strain). This indicates that is present various bacterial species suggests sediments are a natural reservoir gene. Nucleotide sequence revealed two genotypes were found placed genetically distant branches phylogenetic tree, suggesting tet(M)s have different origins.