Controlling Protein Orientation at Interfaces Using Histidine Tags: An Alternative to Ni/NTA

• DOI: 10.1021/ja045084g
• Publication Date: 2005-02-16T13:12:22Z
• Authors: Daniel L. Johnson; Lisandra L. Martin
• Keywords: Models, Molecular; Nitrilotriacetic Acid; Steroid 17-alpha-Hydroxylase; 02 engineering and technology; Surface Plasmon Resonance; Thioredoxins; Nickel; Electrochemistry; Histidine; Gold; Plastocyanin; 0210 nano-technology; Electrodes; Chelating Agents

A strategy for immobilizing histidine-tagged proteins at surfaces has been developed by using a macrocyclic chelator that enhances the stability and specificity of conventional histidine-tag technology allows application this to long-term studies including direct electrochemistry.