Complete Thermal-Unfolding Profiles of Oxidized and Reduced Cytochromes c

• DOI: 10.1021/ja046667t
• Publication Date: 2004-11-10T05:59:55Z
• Authors: Susumu Uchiyama; Atsushi Ohshima; Shota Nakamura; Jun Hasegawa; Norifumi Terui; Shin-ichi Joseph ...; Yasuhiko Yamamoto; Yoshihiro Sambongi; Yuji Kobayashi
• Keywords: Mesophile; Thermus thermophilus; Thermal Stability

The complete thermal-unfolding profiles of both oxidized and reduced forms cytochrome c551 (PA) from mesophilic Pseudomonas aeruginosa c552 (HT) thermophilic Hydrogenobacter thermophilus were obtained by the newly developed pressure-proof cell compartment installed in a circular dichroic spectrometer, which facilitates protein experiments up to 180 °C. thermodynamic cycle, relates stability redox function, indicated that potentials PA HT native state are regulated proteins rather than ones.