Differential Line Broadening in MAS Solid-State NMR due to Dynamic Interference

• DOI: 10.1021/ja072024c
• Publication Date: 2007-07-31T11:00:38Z
• Authors: Veniamin Chevelkov; Katja Faelber; Anna Schrey; Kristina Rehbein; Anne Diehl; Bernd Reif
• Keywords: Line (geometry)

Many MAS (magic angle spinning) solid-state NMR investigations of biologically relevant protein samples are hampered by poor resolution, particularly in the 15N chemical shift dimension. We show that dynamics nanosecond−microsecond time scale can induce significant line broadening resonances experiments. Averaging 15NHα/β multiplet components due to 1H decoupling induces effective relaxation coherence case N−H spin pair undergoes motion. High resolution spectra then only be recorded application TROSY (Transverse Relaxation Optimized Spectroscopy) type techniques which select narrow component pattern. speculate this effect has been major obstacle spectroscopic characterization many membrane proteins and fibrillar aggregates so far. Only very favorable cases, where either absent or fast (picosecond), high-resolution were obtained. expect approach requires intense deuteration will have a impact on quality rate at emerge future.