Development of a Highly Selective Fluorescence Probe for Hydrogen Sulfide

• DOI: 10.1021/ja207851s
• Publication Date: 2011-10-14T20:07:34Z
• Authors: Kiyoshi Sasakura; Kenjiro Hanaoka; Norihiro Shibuya; Yoshinori Mikami; Yuka Kimura; Toru Komatsu; Tasuku Ueno; Takuya Terai; Hideo Kimura; Tetsuo Nagano
• Keywords: Molecular Structure; Organometallic Compounds; Humans; Hydrogen Sulfide; 01 natural sciences; Copper; Fluorescent Dyes; HeLa Cells; 0104 chemical sciences

Hydrogen sulfide (H(2)S) has recently been identified as a biological response modifier. Here, we report the design and synthesis of a novel fluorescence probe for H(2)S, HSip-1, utilizing azamacrocyclic copper(II) ion complex chemistry to control the fluorescence. HSip-1 showed high selectivity and high sensitivity for H(2)S, and its potential for biological applications was confirmed by employing it for fluorescence imaging of H(2)S in live cells.