UV-Induced Bursting of Cell-Sized Multicomponent Lipid Vesicles in a Photosensitive Surfactant Solution

• DOI: 10.1021/ja211664f
• Publication Date: 2012-02-08T18:05:37Z
• Authors: Antoine Diguet; Miho Yanagisawa; Yan-Jun Liu; Elodie Brun; Sacha Abadie; Sergii Rudiuk; Damien Baigl
• Keywords: Bursting; Dipalmitoylphosphatidylcholine; Mole fraction

We study the behavior of multicomponent giant unilamellar vesicles (GUVs) in presence AzoTAB, a photosensitive surfactant. GUVs are made an equimolar ratio dioleoylphosphatidylcholine (DOPC) and dipalmitoylphosphatidylcholine (DPPC) various amounts cholesterol (Chol), where lipid membrane shows phase separation into DPPC-rich liquid-ordered (Lo) DOPC-rich liquid-disordered (Ld) phase. find that UV illumination at 365 nm for 1 s induces bursting significant fraction GUV population. The percentage UV-induced disrupted vesicles, called rate (Yburst), increases with increase [AzoTAB] depends on [Chol] non-monotonous manner. Yburst decreases when from 0 to 10 mol % then further [Chol], which can be correlated composition membrane. show appearance solid domains ([Chol] = 0) or area Lo (with increasing ≥ %). Under our conditions (UV s), maximal efficiency (Yburst 53%) is obtained mM 40 %. Finally, by restricting area, we demonstrate first selective individual target GUVs. These results new method probe biomembrane mechanical properties using light as well pave way novel strategies light-induced drug delivery.