Antibody-Free Reading of the Histone Code Using a Simple Chemical Sensor Array

• DOI: 10.1021/ja303465x
• Publication Date: 2012-06-15T01:50:32Z
• Authors: Samuel A. Minaker; Kevin D. Daze; Manuel C. F. Ma; Fraser Hof
• Keywords: Sequence (biology); Histone code; Identification

The histone code refers to the complex network of post-translational modifications that control gene expression and are high interest as drivers a large number human diseases. We report here on mix-and-match toolkit readily available dyes calixarene host molecules can be combined form dye-displacement sensors respond wide variety cationic peptides. Using data from only two or three such simple supramolecular chemical sensor array produces fingerprints discriminate robustly among many kinds elements. "Reads" accomplished include discrimination unmethylated, mono-, di-, trimethylated lysines single tail sequence, identification different combinations modification type in several sequence contexts, isomeric dimethylarginine modifications. Reads sometimes troublesome for antibodies achieved. also ability simultaneously concentrations identities This discriminates between post-translationally modified analytes without being limited partners contain single, programmed binding interaction.