Highly Specific and Sensitive Electrochemical Genotyping via Gap Ligation Reaction and Surface Hybridization Detection

• DOI: 10.1021/ja808700d
• Publication Date: 2009-01-29T22:04:57Z
• Authors: Yong Huang; Yan-Li Zhang; Xiangmin Xu; Jian-Hui Jiang; Guo-Li Shen; Ru-Qin Yu
• Keywords: Genotype; Beta-Globulins; Nucleic Acid Hybridization; DNA; Electrochemical Techniques; Polymorphism, Single Nucleotide; Sensitivity and Specificity; 01 natural sciences; 0104 chemical sciences; Humans; Gold; Electrodes; Oxidation-Reduction; Alleles

This paper developed a novel electrochemical genotyping strategy based on gap ligation reaction with surface hybridization detection. utilized homogeneous enzymatic reactions to generate molecular beacon-structured allele-specific products that could be cooperatively annealed capture probes stably immobilized the via disulfide anchors, thus allowing ultrasensitive detection of through redox tags in close proximity electrode. Such unique biphasic architecture provided universal methodology for incorporating discrimination desirable reproducibility, high efficiency and no interferences from interficial steric hindrance. The technique was demonstrated show intrinsic sensitivity direct genomic analysis, excellent specificity discriminativity single nucleotide variations.