Engineering Fe–N Doped Graphene to Mimic Biological Functions of NADPH Oxidase in Cells

• DOI: 10.1021/jacs.0c08360
• Publication Date: 2020-10-27T18:22:23Z
• Authors: Di Wu; Jingkun Li; Shujuan Xu; Qianqian Xie; Yanxia Pan; Xi Liu; Ronglin Ma; Huizhen Zheng; Meng Gao; Weili Wang; Jia Li; Xiaoming Cai; Frédéric Jaouen; Ruibin Li
• Keywords: Chronic Granulomatous Disease

NADPH oxidase (NOX) as a transmembrane enzyme complex controls the generation of superoxide that plays important roles in immune signaling pathway. NOX inactivation may elicit immunodeficiency and cause chronic granulomatous disease (CGD). Biocompatible synthetic materials with NOX-like activities would therefore be interesting curative and/or preventive approaches case deficiency. Herein, we synthesized Fe–N doped graphene (FeNGR) nanomaterial could mimic activity by efficiently catalyzing conversion into NADP+ triggering oxygen radicals. The resulting FeNGR nanozyme had similar cellular distribution to is able function NOX-deficient cells retrieving activity, evidenced TNF-α, IL-1β, IL-6 production response Alum exposure. Overall, our study discovered material demonstrated its biological activation cells.