Engineering Fe–N Doped Graphene to Mimic Biological Functions of NADPH Oxidase in Cells
Models, Molecular
Nitrogen
THP-1 Cells
Iron
Interleukin-1beta
01 natural sciences
Biomimetic Materials
Superoxides
Doping
Humans
[SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM]
Fluorescent Dyes
Peroxidase
[CHIM.MATE] Chemical Sciences/Material chemistry
NADPH oxidase
Interleukin-6
Tumor Necrosis Factor-alpha
Nanozyme
[CHIM.CATA] Chemical Sciences/Catalysis
NADPH Oxidases
0104 chemical sciences
Nanocatalyst
Graphite
Graphene
Reactive Oxygen Species
Oxidation-Reduction
NADP
Signal Transduction
DOI:
10.1021/jacs.0c08360
Publication Date:
2020-10-27T18:22:23Z
AUTHORS (14)
ABSTRACT
NADPH oxidase (NOX) as a transmembrane enzyme complex controls the generation of superoxide that plays important roles in immune signaling pathway. NOX inactivation may elicit immunodeficiency and cause chronic granulomatous disease (CGD). Biocompatible synthetic materials with NOX-like activities would therefore be interesting as curative and/or preventive approaches in case of NOX deficiency. Herein, we synthesized a Fe-N doped graphene (FeNGR) nanomaterial that could mimic the activity of NOX by efficiently catalyzing the conversion of NADPH into NADP+ and triggering the generation of oxygen radicals. The resulting FeNGR nanozyme had similar cellular distribution to NOX and is able to mimic the enzyme function in NOX-deficient cells by catalyzing the generation of superoxide and retrieving the immune activity, evidenced by TNF-α, IL-1β, and IL-6 production in response to Alum exposure. Overall, our study discovered a synthetic material (FeNGR) to mimic NOX and demonstrated its biological function in immune activation of NOX-deficient cells.
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