Engineering Fe–N Doped Graphene to Mimic Biological Functions of NADPH Oxidase in Cells

Models, Molecular Nitrogen THP-1 Cells Iron Interleukin-1beta 01 natural sciences Biomimetic Materials Superoxides Doping Humans [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] Fluorescent Dyes Peroxidase [CHIM.MATE] Chemical Sciences/Material chemistry NADPH oxidase Interleukin-6 Tumor Necrosis Factor-alpha Nanozyme [CHIM.CATA] Chemical Sciences/Catalysis NADPH Oxidases 0104 chemical sciences Nanocatalyst Graphite Graphene Reactive Oxygen Species Oxidation-Reduction NADP Signal Transduction
DOI: 10.1021/jacs.0c08360 Publication Date: 2020-10-27T18:22:23Z
ABSTRACT
NADPH oxidase (NOX) as a transmembrane enzyme complex controls the generation of superoxide that plays important roles in immune signaling pathway. NOX inactivation may elicit immunodeficiency and cause chronic granulomatous disease (CGD). Biocompatible synthetic materials with NOX-like activities would therefore be interesting as curative and/or preventive approaches in case of NOX deficiency. Herein, we synthesized a Fe-N doped graphene (FeNGR) nanomaterial that could mimic the activity of NOX by efficiently catalyzing the conversion of NADPH into NADP+ and triggering the generation of oxygen radicals. The resulting FeNGR nanozyme had similar cellular distribution to NOX and is able to mimic the enzyme function in NOX-deficient cells by catalyzing the generation of superoxide and retrieving the immune activity, evidenced by TNF-α, IL-1β, and IL-6 production in response to Alum exposure. Overall, our study discovered a synthetic material (FeNGR) to mimic NOX and demonstrated its biological function in immune activation of NOX-deficient cells.
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