A Bioorthogonal Probe for Multiscale Imaging by 19F-MRI and Raman Microscopy: From Whole Body to Single Cells

• DOI: 10.1021/jacs.1c05250
• Publication Date: 2021-07-28T22:11:05Z
• Authors: Cristina Chirizzi; Carlo Morasso; Alessandro Aldo C...; Matteo Tommasini; Fabio Corsi; Linda Chaabane; Renzo Vanna; Francesca Baldell...; Pierangelo Metran...
• Keywords: Bioorthogonal Chemistry; Molecular Imaging; Ex vivo; Direct imaging; Raman microspectroscopy; Molecular probe

Molecular imaging techniques are essential tools for better investigating biological processes and detecting disease biomarkers with improvement of both diagnosis therapy monitoring. Often, a single technique is not sufficient to obtain comprehensive information at different levels. Multimodal diagnostic probes key enable across multiple scales. The direct registration in vivo markers ex the cellular level probe still challenging. Fluorinated (19F) have been increasingly showing promising potentialities cell tracking by 19F-MRI. Here we present unique features bioorthogonal 19F-probe that enables signal correlation MRI Raman imaging. In particular, reveal ability PERFECTA, superfluorinated molecule, exhibit remarkable intense distinct from tissue fingerprints. Therefore, PERFECTA combines molecule excellent characteristics macroscopic 19F-MRI, whole body, microscopic levels