Programming 2D Supramolecular Assemblies with Wireframe DNA Origami

• DOI: 10.1021/jacs.1c11332
• Publication Date: 2022-03-01T16:55:05Z
• Authors: Xiao Wang; Hyungmin Jun; Mark Bathe
• Keywords: DNA origami; DNA nanotechnology; Morphing

Wireframe DNA origami offers the ability to program nearly arbitrary 2D and 3D nanoscale geometries, with six-helix bundle (6HB) edge designs providing both geometric versatility fidelity respect target shape. Because individual objects are limited in size by length of scaffold, here, we introduce a hierarchical self-assembly strategy overcome this limitation programming supramolecular assemblies periodic arrays using wireframe as building blocks. Parallel half-crossovers used together lateral cohesive interactions between staples scaffold symmetry into constructed from single units that cannot be self-assembled directly base-stacking or conventional antiparallel crossover designs. This design approach can applied readily designed top-down sequence METIS without any prerequisites on staple routing. We demonstrate utility our fabricating dimers self-limiting hexameric superstructures triangular hexagonal generalize fabricate close-packed non-close-packed arrays. Visualization atomic force microscopy transmission electron demonstrates exhibit similar structural integrity blocks METIS. Our results offer general platform for fabrication materials variety applications.