DNA Cryogels with Anisotropic Mechanical and Responsive Properties for Specific Cell Capture and Release

• DOI: 10.1021/jacs.3c12846
• Publication Date: 2024-02-21T04:53:42Z
• Authors: Yali Mu; Xiaowen Wang; Xiaoxue Du; Ping-Ping He; Weiwei Guo
• Keywords: Biocompatibility

Due to their programmable stimuli-responsiveness, excellent biocompatibility, and water-rich soft structures similar biological tissues, smart DNA hydrogels hold great promise for biosensing biomedical applications. However, most developed date are composed of randomly oriented isotropic polymer networks, the resulting slow response biotargets lack anisotropic properties those tissues have limited extensive Herein, consisting unidirectional void channels internally up macroscopic length scales were constructed by a directional cryopolymerization method, as exemplified DNA-incorporated covalently cross-linked cryogel duplex structure noncovalently cryogel. Results showed that formation significantly improved responsiveness gel matrix biomacromolecular substances further endowed cryogels with properties, including mechanical swelling/shrinking behaviors, specific biotargets. Moreover, abundant long macroporous in facilitated migration cells, through introduction aptamer thermosensitive polymers, an cryogel-based platform was achieve highly efficient capture release cells. These may provide new opportunities development separation systems.