Near-Infrared Perylenecarboximide Fluorophores for Live-Cell Super-Resolution Imaging

• DOI: 10.1021/jacs.3c13368
• Publication Date: 2024-03-05T16:26:39Z
• Authors: Ze-Hua Wu; Xingfu Zhu; Qiqi Yang; Yulian Zagranyarski; Krishna Mishra; Hilmar Strickfaden; Ronald P. Wong; Thomas Basché; Kaloian Koynov; Mischa Bonn; Chen Li; Xiaomin Liu; Klaus Müllen
• Keywords: Chromophore

Organic near-infrared (NIR) photoblinking fluorophores are highly desirable for live-cell super-resolution imaging based on single-molecule localization microscopy (SMLM). Herein we introduce a novel small chromophore, PMIP, through the fusion of perylenecarboximide with 2,2-dimetheylpyrimidine. PMIP exhibits an emission maximum at 732 nm high fluorescence quantum yield 60% in wavelength range 700–1000 and excellent without any additives. With resorcinol-functionalized (PMIP-OH), NIR SMLM lysosomes is demonstrated first time living mammalian cells under physiological conditions. Moreover, metabolically labeled nascent DNA site-specifically detected using azido-functionalized (PMIP-N3) via click chemistry, thereby enabling phosphate-buffered saline 9-fold improvement spatial resolution. These results indicate potential PMIP-based blinking biological applications SMLM.