In Vivo Fluorescent Labeling of Foam Cell-Derived Extracellular Vesicles as Circulating Biomarkers for In Vitro Detection of Atherosclerosis

• DOI: 10.1021/jacs.4c01173
• Publication Date: 2024-03-28T11:02:09Z
• Authors: Moxuan Ji; Yongchun Wei; Zhuo Ye; Xiaoqin Hong; Xiaoxuan Yu; Rui Du; Qiang Li; Wei Sun; Dingbin Liu
• Keywords: Nanoprobe; Ex vivo; Foam cell

Real-time monitoring of the development atherosclerosis (AS) is key to management cardiovascular disease (CVD). However, existing laboratory approaches lack sensitivity and specificity, mostly due dearth reliable AS biomarkers. Herein, we developed an in vivo fluorescent labeling strategy that allows specific staining foam cell-derived extracellular vesicles (EVs) atherosclerotic plaques, which are released into blood as circulating biomarkers for vitro detection AS. This relies on a self-assembled nanoprobe could recognize cells specifically, where probe degraded by intracellular HClO produce trifluoromethyl-bearing boron-dipyrromethene fluorophore (termed B-CF3), lipophilic dye can be transferred exosomal membranes. These B-CF3-stained EVs detected directly fluorescence spectrometer or microplate reader without resorting any sophisticated analytical method. liquid-biopsy format enables early real-time differentiation lesion vulnerability during progression, facilitating effective CVD management.