Random Sanitization in DNA Information Storage Using CRISPR-Cas12a

• DOI: 10.1021/jacs.4c11380
• Publication Date: 2024-12-11T03:42:11Z
• Authors: Hongyu Shen; Zhi Weng; Haipei Zhao; Haitao Song; Fei Wang; Chunhai Fan; Ping Song

DNA information storage provides an excellent solution for metadata due to its high density, programmability, and long-term stability. However, current research primarily focuses on the processes of storing reading data, lacking comprehensive solutions secure wiping. Herein, we present a method random sanitization in using CRISPR-Cas12a (RSDISC) based precise control thermodynamic energy primer-template hybridization. We utilize collateral cleavage (trans-activity) single-stranded (ssDNA) by achieve selective files metadata. This enables ssDNA degradation with different GC contents, lengths, secondary structures efficiency up 99.9% 28,258 oligonucleotides within one round. demonstrate that number erasable could reach 10