Liquid Phase Exfoliation of Protein Parent Crystals into Nanosheets and Fibrils Based on Orthogonal Supramolecular Interactions

• DOI: 10.1021/jacs.4c11921
• Publication Date: 2024-11-12T14:19:50Z
• Authors: Long Li; Linfei Ye; Yiwei Shi; Lin Yin; Guosong Chen
• Keywords: Exfoliation joint

Proteins are attractive building blocks for fabricating diverse and precise nanomaterials. However, the facile fabrication of multidimensional artificial assemblies is highly challenging. Here, inspired by large-scale production technique inorganic nanomaterials, we demonstrate application liquid phase exfoliation (LPE) on native protein ConA design synthetic ligands. These ligands provide distinct in-plane out-of-plane supramolecular interactions, allowing generation architectures based same dissociating a single interaction in solution, including 3D porous crystals, 2D sizable nanosheets, 1D fibrils. Importantly, exfoliated sheets were dozens times larger than self-assembled resulting dramatic enhancement intrinsic bioactivity receptor clustering less endocytosis. findings enable successful LPE biomacromolecules open up an alternative avenue to generate advanced without need complex careful adjustment self-assembly conditions.