Directed Assembly of Nucleic Acid-Based Polymeric Nanoparticles from Molecular Tetravalent Cores

• DOI: 10.1021/jacs.5b03485
• Publication Date: 2015-05-17T20:06:04Z
• Authors: Bong Jin Hong; Ibrahim Eryazici; Reiner Bleher; Ryan V. Thaner; Chad A. Mirkin; SonBinh T. Nguyen
• Keywords: Surface Modification

Complementary tetrahedral small molecule-DNA hybrid (SMDH) building blocks have been combined to form nucleic acid-based polymeric nanoparticles without the need for an underlying template or scaffold. The sizes of these particles can be tailored in a facile fashion by adjusting assembly conditions such as SMDH concentration, time, and NaCl concentration. Notably, novel stabilized transformed into functionalized spherical acid (SNA) structures through incorporation capping DNA strands conjugated with functional groups. These results demonstrate systematic, efficient strategy construction surface functionalization well-defined, size-tunable from readily accessible molecular blocks. Furthermore, because exhibited enhanced cellular internalization resistance DNase I compared free synthetic acids, they should plethora applications diagnostics therapeutics.