Noncovalent Modulation of the Inverse Temperature Transition and Self-Assembly of Elastin-b-Collagen-like Peptide Bioconjugates

• DOI: 10.1021/jacs.5b09941
• Publication Date: 2015-12-03T19:15:04Z
• Authors: Tianzhi Luo; Kristi L. Kiick
• Keywords: Conjugate

Stimuli-responsive nanostructures produced with peptide domains from the extracellular matrix offer great opportunities for imaging and drug delivery. Although individual utility of elastin-like (poly)peptides collagen-like peptides in such applications has been demonstrated, synergistic advantages combining these motifs short conjugates have surprisingly not reported. Here, we introduce conjugation a thermoresponsive (ELP) triple-helix-forming (CLP) to yield ELP–CLP that show remarkable reduction inverse transition temperature ELP domain upon formation CLP triple helix. The lower conjugate enables facile well-defined vesicles at physiological unexpected resolubilization elevated temperatures unfolding domain. Given demonstrated ability CLPs modify collagens, our results only provide simple versatile avenue controlling behavior ELPs, but also suggest future biologically relevant environments.