Ultrasmall Magnetically Engineered Ag2Se Quantum Dots for Instant Efficient Labeling and Whole-Body High-Resolution Multimodal Real-Time Tracking of Cell-Derived Microvesicles

• DOI: 10.1021/jacs.5b10340
• Publication Date: 2016-01-23T16:10:45Z
• Authors: Jing-Ya Zhao; Gang Chen; Yi-Ping Gu; Ran Cui; Zhi-Ling Zhang; Zi-Li Yu; Bo Tang; Yi-Fang Zhao; Dai-Wen Pang
• Keywords: Tracking (education); Instant

Cell-derived microvesicles (MVs) are natural carriers that can transport biological molecules between cells, which expected to be promising delivery vehicles for therapeutic purposes. Strategies label MVs very important investigation and application of MVs. Herein, ultrasmall Mn-magnetofunctionalized Ag2Se quantum dots (Ag2Se@Mn QDs) integrated with excellent near-infrared (NIR) fluorescence magnetic resonance (MR) imaging capabilities have been developed instant efficient labeling their in vivo high-resolution dual-mode tracking. The Ag2Se@Mn QDs were fabricated by controlling the reaction Mn2+ nanocrystals having pretreated 80 °C NaOH solution, an size ca. 1.8 nm, water dispersibility, high NIR yield 13.2%, longitudinal relaxivity 12.87 mM–1 s–1 (almost four times commercial contrast agent Gd-DTPA). enables them directly efficiently loaded into electroporation, instantly reliably conferring both MR traceability on Our method different origins is universal free unfavorable influence intrinsic behaviors complementary made long-term noninvasive whole-body tracking realized, dynamic biodistribution has revealed a real-time situ quantitative manner. This work not only opens new window QDs, but also facilitates greatly