Monodisperse Uni- and Multicompartment Liposomes

• DOI: 10.1021/jacs.6b02107
• Publication Date: 2016-05-31T21:36:05Z
• Authors: Nan-Nan Deng; Maaruthy Yelleswa...; Wilhelm T. S. Huck
• Keywords: Dispersity; Artificial cell

Liposomes are self-assembled phospholipid vesicles with great potential in fields ranging from targeted drug delivery to artificial cells. The formation of liposomes using microfluidic techniques has seen considerable progress, but the process itself not been studied detail. As a result, high throughput, high-yielding routes monodisperse multiple compartments have demonstrated. Here, we report on surfactant-assisted route uniform, single bilayer liposomes, 25 190 μm, and or without inner compartments. key our method is precise control over developing interfacial energies complex W/O/W emulsion systems during liposome formation, which achieved via an additional surfactant outer water phase. consist bilayers, as demonstrated by nanopore experiments confocal fluorescence microscopy, they can act for cell-free gene expression. technique be expanded create multitude coupled compartments, opening networks multistep microreactors.