Highly Sensitive Coherent Anti-Stokes Raman Scattering Imaging of Protein Crystals

0301 basic medicine 03 medical and health sciences
DOI: 10.1021/jacs.6b04464 Publication Date: 2016-09-16T19:23:12Z
ABSTRACT
Serial crystallography at last generation X-ray synchrotron sources and free electron lasers enabled data collection with micrometer and even submicrometer size crystals, which have resulted in amazing progress in structural biology. However, imaging of small crystals, which although is highly demanded, remains a challenge, especially in the case of membrane protein crystals. Here we describe a new extremely sensitive method of the imaging of protein crystals that is based on coherent anti-Stokes Raman scattering.
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