Highly Sensitive Coherent Anti-Stokes Raman Scattering Imaging of Protein Crystals

• DOI: 10.1021/jacs.6b04464
• Publication Date: 2016-09-16T19:23:12Z
• Authors: Grigory M. Arzuma...; Nelya V. Doroshke...; Kahramon Z. Mamat...; Sergey N. Shashkov; Egor V. Zinovev; Alexey V. Vlasov; Ekaterina S. Round; Valentin I. Gordeliy
• Keywords: Protein crystallization; Electron crystallography; Micrometer

Serial crystallography at last generation X-ray synchrotron sources and free electron lasers enabled data collection with micrometer even submicrometer size crystals, which have resulted in amazing progress structural biology. However, imaging of small although is highly demanded, remains a challenge, especially the case membrane protein crystals. Here we describe new extremely sensitive method crystals that based on coherent anti-Stokes Raman scattering.