Fluorogenic Probes for Multicolor Imaging in Living Cells

• DOI: 10.1021/jacs.6b04782
• Publication Date: 2016-07-15T21:14:44Z
• Authors: Gražvydas Lukinav...; Luc Reymond; Keitaro Umezawa; Olivier Sallin; Elisa D’Este; Fabian Göttfert; Haisen Ta; Stefan W. Hell; Yasuteru Urano; Kai Johnsson
• Keywords: Live cell imaging; Rhodamine; Fluorescence-lifetime imaging microscopy

Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 715 nm, respectively. SiR700-based probes F-actin, microtubules, lysosomes, SNAP-tag are fluorogenic, cell-permeable, compatible with superresolution microscopy. In conjunction based on the previously introduced carboxy-SiR650, permit microscopy in thus significantly expanding our capacity imaging living cells.