Defining Noncovalent Ubiquitin Homodimer Interfacial Interactions through Comparisons with Covalently Linked Diubiquitin

• DOI: 10.1021/jacs.6b09829
• Publication Date: 2016-12-15T00:26:27Z
• Authors: Nicole D. Wagner; David H. Russell
• Keywords: Non-covalent interactions; Hydrophobic effect; Static electricity

Covalently linked diubiquitin (diUbq) is known to adopt specific interfacial interactions owing steric hindrance induced by the covalent tether. K48-linked diUbq preferentially forms hydrophobic between two I44 faces under physiological conditions, whereas K63-linked electrostatic interactions. Here, we show using collision-induced unfolding ion mobility-mass spectrometry that recently reported noncovalent dimer of ubiquitin exhibits structural preferences and are most similar diUbq.