Mapping of Molecular Structure of the Nanoscale Surface in Bionanoparticles

• DOI: 10.1021/jacs.6b12297
• Publication Date: 2016-12-24T23:04:24Z
• Authors: Luciana M. Herda; Delyan R. Hristov; Maria Cristina Lo...; Ester Polo; Kenneth A. Dawson
• Keywords: Immunogold labelling; Nanomaterials

Characterizing the orientation of covalently conjugated proteins on nanoparticles, produced for in vitro and vivo targeting, though an important feature such a system, has proved challenging. Although extensive physicochemical characterization targeting nanoparticles can be addressed detail, relevant biological nanointerface is crucial order to select suitable nanomaterials further or experiments. In this work, we adopt methodology using antibody fragments (Fab) gold (immunogold) map available epitopes transferrin grafted silica particle (SiO2-PEG8-Tf) as proxy predict nanoparticle function, therefore cellular receptor engagement. Data from adopted method suggest that, average, only ∼3.5% SiO2-PEG8-Tf surface have favorable recognition by receptor.