Photochemical Barcodes

• DOI: 10.1021/jacs.8b00887
• Publication Date: 2018-03-21T17:39:15Z
• Authors: Sicheng Tang; Yang Zhang; Pravat Dhakal; Laura Ravelo; Cheyenne L. Anderson; Kevin M. Collins; Françisco M. Raymo
• Keywords: BODIPY

A photochemical strategy to encode fluorescence signals in vivo with spatial control was designed around the unique properties of a photoactivatable borondipyrromethene (BODIPY). The photoinduced disconnection two oxazines, flanking single BODIPY, consecutive steps produces mixture three emissive molecules resolved inside polymer beads. relative amounts and emission intensities fluorophores can be regulated precisely each bead by adjusting dose activating photons mark individual particles distinct codes signals. visible wavelengths mild illumination sufficient induce these transformations permit barcoding beads also living nematodes. Different regions same animal labeled barcodes allow monitoring their dynamics for long times no toxic effects. Thus, our generation produce multiple distinguishable labels biological sample enable spatiotemporal tracking of, otherwise indistinguishable, targets.