Intercommunication of DNA-Based Constitutional Dynamic Networks

• DOI: 10.1021/jacs.8b03450
• Publication Date: 2018-07-02T19:31:26Z
• Authors: Liang Yue; Shan Wang; Sivan Lilienthal; Verena Wulf; Françoise Remacle; R. D. Levine; Itamar Willner
• Keywords: Computers, Molecular; Cations, Divalent; Biocatalysis; Magnesium; DNA, Catalytic; 02 engineering and technology; 0210 nano-technology; 01 natural sciences; Fluorescent Dyes; 0104 chemical sciences

Intercommunication between dynamic chemical networks plays a major role in cellular transformations. Inspired by nature, we introduce the intercommunication two constitutional networks, CDNs, "S" and "T" composed, each, of four equilibrated supramolecular constituents AA′, AB′, BA′, BB′, CC′, CD′, DC′, DD′, respectively. Each is conjugated to Mg2+-ion-dependent DNAzyme unit that acts as reporter element for concentration respective constituent via catalyzed cleavage fluorophore/quencher-functionalized substrate associated with reporter. Also, BB′ (in CDN "S") CC′ "T") include DNAzymes acting activator units generating triggering signals networks. Subjecting CDNs catalytically cleavable hairpin trigger Hdd′ or Haa′, respectively, yields input strands intercommunicate affecting time-dependent re-equilibration counter without equilibrium generates strands. Treatment hairpins Haa′ (or Hba′), stimulates autonomous positive/positive positive/negative feedback programmed up-regulation down-regulation CDNs.