Photoexcitation of the P4480 State Induces a Secondary Photocycle That Potentially Desensitizes Channelrhodopsin-2
Neurons
0301 basic medicine
0303 health sciences
Light
500 Naturwissenschaften und Mathematik::530 Physik::530 Physik
photonics
530
01 natural sciences
0104 chemical sciences
Kinetics
03 medical and health sciences
Channelrhodopsins
photoexcitation
kinetics lasers
light
DOI:
10.1021/jacs.8b03931
Publication Date:
2018-07-23T20:49:50Z
AUTHORS (6)
ABSTRACT
Channelrhodopsins (ChRs) are light-gated cation channels. In spite of their wide use to activate neurons with light, the photocurrents of ChRs rapidly decay in intensity under both continuous illumination and fast trains of light pulses, broadly referred to as desensitization. This undesirable phenomenon has been explained by two interconnected photocycles, each of them containing a nonconductive dark state (D1 and D2) and a conductive state (O1 and O2). While the D1 and O1 states correspond to the dark-state and P3520 intermediate of the primary all-trans photocycle of ChR2, the molecular identity of D2 and O2 remains unclear. We show that P4480, the last intermediate of the all-trans photocycle, is photoactive. Its photocycle, characterized by time-resolved UV/vis spectroscopy, contains a red-shifted intermediate, I3530. Our results indicate that the D2 and O2 states correspond to the P4480 and I3530 intermediates, connecting desensitization of ChR2 with the photochemical properties of the P4480 intermediate.
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