An ESIPT Probe for the Ratiometric Imaging of Peroxynitrite Facilitated by Binding to Aβ-Aggregates

• DOI: 10.1021/jacs.8b08457
• Publication Date: 2018-10-02T16:21:35Z
• Authors: Adam C. Sedgwick; Wei-Tao Dou; Jin-Biao Jiao; Luling Wu; George T. Williams; A. Toby A. Jenkins; Steven D. Bull; Jonathan L. Sessler; Xiao-Peng He; Tony D. James
• Keywords: Flavonoids; Amyloid beta-Peptides; Molecular Structure; Optical Imaging; Mice, Transgenic; 540; 01 natural sciences; 543; 0104 chemical sciences; 3. Good health; Mice; Protein Aggregates; Peroxynitrous Acid; Animals; Protons; Fluorescent Dyes

A series of 3-hydroxyflavone (3-HF) ESIPT (excited-state intramolecular proton transfer) boronate-based fluorescent probes have been developed for the detection peroxynitrite (ONOO–). The dyes are environmentally sensitive, and each probe exhibited a ratiometric response toward ONOO– in micellar environment. were used to image different aggregation states amyloid-β (Aβ) presence ONOO–. 3-HF-OMe was found produce when bound Aβ aggregates, resulting novel host–guest ensemble, which adds insight into development other ESIPT-based simultaneous sensing fibrous proteins/peptides environmental ROS/RNS.