Identification of the Formycin A Biosynthetic Gene Cluster from Streptomyces kaniharaensis Illustrates the Interplay between Biological Pyrazolopyrimidine Formation and de Novo Purine Biosynthesis

• DOI: 10.1021/jacs.9b00241
• Publication Date: 2019-04-03T15:38:36Z
• Authors: Shao-An Wang; Yeonjin Ko; Jia Zeng; Yujie Geng; Daan Ren; Yasushi Ogasawara; Seema Irani; Yan Zhang; Hung-wen Liu
• Keywords: Gene cluster; Secondary metabolism

Formycin A is a potent purine nucleoside antibiotic with C-glycosidic linkage between the ribosyl moiety and pyrazolopyrimidine base. Herein, cosmid identified from Streptomyces kaniharaensis genome library that contains for gene cluster responsible biosynthesis of formycin. Subsequent deletion experiments in vitro characterization forBCH products established their catalytic functions formycin biosynthesis. Results also demonstrated PurH de novo plays key role formation during A. The participation both pathways represents good example how primary secondary metabolism are interlinked.